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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been noticed in several tumors, which ends up in chromosome instability, and so might add to tumorigenesis. This protein was identified to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a crucial position in advertising and marketing mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have been described. Two extra splice variants were being noted but thorough nucleotide sequence details just isn't still readily available.
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Blocking peptides are peptides that bind specifically to the concentrate on antibody and block antibody binding. These peptide typically includes the epitope acknowledged through the antibody.
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This guidebook outlines further doable optimization of cellular and immunoassay parameters to be sure the best possible effects are...
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This guide outlines more doable optimization of cellular and immunoassay parameters to be certain the best possible results are...
Antibodies bound to the blocking peptide not bind to your epitope to the concentrate on protein. By evaluating the staining from your blocked antibody as opposed to the antibody by itself, you can see which staining is precise.
Concentrate on Facts Cyclin E belongs for the extremely conserved cyclin family members, whose members are characterised by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins perform as regulators of CDK kinases. Diverse cyclins exhibit distinct expression and degradation patterns which contribute into the temporal coordination of each mitotic event. Cyclin E types a posh with and functions as being a regulatory subunit of CDK2, whose activity is required for mobile cycle G1/S changeover. This protein accumulates with the G1-S period boundary and is degraded as cells progress through S section.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...
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